Question: Consider a simple pH indicator dye that changes color with pH. Let's suppose that our dye absorbs strongly at a given wavelength at pH 12
Consider a simple pH indicator dye that changes color with pH. Let's suppose that our dye absorbs strongly at a given wavelength at pH 12 while it has essentially no absorbance at pH 2 as shown in the table below dye concentration pH Abs 10 micromolar 2 0.00 10 micromolar 12 0.90 At pH 7 you observe the absorbance of your 10 micromolar solution to be 0.3 Calculate the pKa to 2 significant figures You can assume that our dye is a simple monoprotic acid that is fully deprotonated at pH 12 and fully protonated at pH 2.
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