Question: How can I construct a hyperbolic Michaelis-Menten plots on excel using the data on the table Thank you Varying ONPG, ONPG stock solution = 5
How can I construct a hyperbolic Michaelis-Menten plots on excel using the data on the table
Thank you
| Varying ONPG, ONPG stock solution = 5 mg/mL | |||||||||||
| Lactase Vol (uL) | ONPG Vol (uL) | Tris Buffer Vol (mL) | Total Vol (mL) | Start Abs | 15" Abs | 30" Abs | 45" Abs | 60" Abs | 75" Abs | 95" Abs | 105" Abs |
| 100 | 25 | 5.88 | 6.00 | 0.000 | 0.025 | 0.050 | 0.074 | 0.100 | 0.125 | 0.159 | 0.173 |
| 100 | 35 | 5.87 | 6.00 | 0.000 | 0.032 | 0.063 | 0.095 | 0.127 | 0.161 | 0.202 | 0.225 |
| 100 | 50 | 5.85 | 6.00 | 0.000 | 0.041 | 0.081 | 0.121 | 0.164 | 0.204 | 0.258 | 0.283 |
| 100 | 100 | 5.80 | 6.00 | 0.000 | 0.059 | 0.119 | 0.179 | 0.239 | 0.299 | 0.377 | 0.422 |
| 100 | 150 | 5.75 | 6.00 | 0.000 | 0.070 | 0.142 | 0.211 | 0.283 | 0.354 | 0.443 | 0.493 |
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