Question: How can I construct a hyperbolic Michaelis-Menten plots on excel using the data on the table Thank you Varying ONPG, ONPG stock solution = 5

How can I construct a hyperbolic Michaelis-Menten plots on excel using the data on the table

Thank you

Varying ONPG, ONPG stock solution = 5 mg/mL

Lactase Vol (uL)

ONPG Vol (uL)

Tris Buffer Vol (mL)

Total Vol (mL)

Start Abs

15" Abs

30" Abs

45" Abs

60" Abs

75" Abs

95" Abs

105" Abs

100

25

5.88

6.00

0.000

0.025

0.050

0.074

0.100

0.125

0.159

0.173

100

35

5.87

6.00

0.000

0.032

0.063

0.095

0.127

0.161

0.202

0.225

100

50

5.85

6.00

0.000

0.041

0.081

0.121

0.164

0.204

0.258

0.283

100

100

5.80

6.00

0.000

0.059

0.119

0.179

0.239

0.299

0.377

0.422

100

150

5.75

6.00

0.000

0.070

0.142

0.211

0.283

0.354

0.443

0.493

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