Question: How do you calculate the concentrations using the absorbances? Penicillin G reacts with hydroxylamine (NO2OH) according to the following reaction: P + NH2OH NH2OHP to
Penicillin G reacts with hydroxylamine (NO2OH) according to the following reaction: P + NH2OH NH2OHP to form hydroxamic acid, which produces a colored complex with Fe(III). To determine the overall reaction order, equal concentrations of penicillin (P) and hydroxylamine (NO2OH) were mixed together in a 250 ml flask [J. Chem. Educ., 49, 539 (1972)]. Samples were withdrawn every 10 minutes and added to a solution containing iron (III) chloride. Absorbance measurements obtained with the aid of a colorimeter were obtained as a function of time. The absorbance shown in the table below is known to be directly proportional to the hydroxamic acid (NH2OHP) concentration. Run 1 2 3 4 5 6 7 time, min 0 10 20 30 40 50 1000 (or ) Absorbance 00.34 0.43 0.5 0.54 0.56 0.69 Hint: Notice you can build a linear calibration between Absorbance (A) and conversion (xA); i.e. XA, 0-1(1, 0 ) while A, 0 0.69 Using both (the value at t is provided as a reference for calibration only, not to be used in the kinetic analysis)
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