Question: Make 1L of a 1X Buffer A solution. Below is the formulation for the 1X Buffer A: 1X Buffer A Information given 10 mM Tris-HCI,

 Make 1L of a 1X Buffer A solution. Below is the

Make 1L of a 1X Buffer A solution. Below is the formulation for the 1X Buffer A: 1X Buffer A Information given 10 mM Tris-HCI, PH 7.5 MW 121.1 1 mM EDTA You have a 1M stock 5 mM beta-mercaptoethanol (BME) You have a 1M stock 0.5% v/v glycerol (glycerol is a liquid; it is 100% v/v) Chose the correct amounts of each of the components to use to make 1 of 1X Buffer A. 1.21g Tris, 1mL EDTA, 5mL BME, 5mL Glycerol 1.21g Tris, 1mL EDTA, 5mL BME, 10mL Glycerol 24.22g Tris, 20mL EDTA, 100mL BME, 100mL Glycerol 24.22g Tris, 20mL EDTA, 50mL BME, 50mL Glycerol

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer
Step: 1 Unlock blur-text-image
Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock
Step: 3 Unlock

Students Have Also Explored These Related Chemical Engineering Questions!