Test your knowledge-Practice Problems:\ Dilution 1: You want to make

100ml

of

1\\\\times

TAE buffer from a

20x

TAE solution. Determine how much stock buffer and water you will need to make the dilute buffer. Calculate the dilution factor.\

1\\\\times

TAE\

V_(1)=

volume of stock solution needed to make dilute solution =\

C_(1)=

concentration of stock solution

=

\

V_(2)=

final volume of dilute solution =\

C_(2)=

final concentration of dilute solution

=

\ Calculate

V_(1)

:\ Calculate

V_(water: )

\

DF=

\ Dilution 2: You have a

10m(g)/(m)l

stock solution of ethidium bromide and want a final concentration of

1\\\\mu (g)/(m)l

in

1L

of premade

1x

TAE buffer. Determine how much stock ethidium bromide and 1X TAE you will need to make the dilute solution. Calculate the dilution factor.\

V_(1)=

volume of stock solution needed to make dilute solution =\

C_(1)=

concentration of stock solution

=

\

V_(2)=

final volume of dilute solution

=

\

C_(2)=

final concentration of dilute solution

=

\ Calculate

V_(1)

:\ Calculate

V_(1\\\\times TAE)

\

DF=

V1= volume of stock solution needed to make dilute solution = C1= concentration of stock solution = V2= final volume of dilute solution = C2= final concentration of dilute solution = Calculate V1 : Calculate Vwater: DF= Dilution 2: You have a 10mg/ml stock solution of ethidium bromide and want a final concentration of 1g/ml in 1L of premade 1 X TAE buffer. Determine how much stock ethidium bromide and 1X TAE you will need to make the dilute solution. Calculate the dilution factor. V1= volume of stock solution needed to make dilute solution = C1= concentration of stock solution = V2= final volume of dilute solution = C2= final concentration of dilute solution = Calculate V1 : Calculate V1XTAE