Question: Module III: Genomic DNA Extraction Performance Indicators: Geneticists will be able to extract high purity genomic DNA from Aedes aegypii using Zymo BashingBead Protocol Geneticists

Module III: Genomic DNA Extraction Performance Indicators: Geneticists will be able to extract high purity genomic DNA from Aedes aegypii using Zymo BashingBead Protocol Geneticists will be able to identify physical and chemical properties of DNA molecules. Guided Notes: Your notebook should contain information sufficient to respond to these questions. What property or properties of DNA are utilized to perform a high purity genomic DNA extraction? Why is it necessary to use elution buffer in the last step of our extraction? During which points in this process are we using physical processes to purify our DNA? When are we using chemical processes? How does DNA behave when it is exposed to an electric field? Why does it behave this way? Identify the role of the following reagents/buffers in this extraction protocol. BashingBead Buffer: Genomic Lysis Buffer: DNA Pre-Wash Buffer: g-DNA Wash Buffer: Elution Buffer:

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