Question: Must put it on excel, include functions and equations. In biochemistry, Michaelis-Menton kinetics is one of the simplest and best-known models of enzyme kinetics. The
Must put it on excel, include functions and equations.
- In biochemistry, Michaelis-Menton kinetics is one of the simplest and best-known models of enzyme kinetics. The model takes the form of an equation describing the rate of enzymatic reactions, by relating rate v to [S], the concentration of substrate S. Its formula is given by
v= Vmax[S]/Km+[S]
Here, Vmax represents the maximum rate achieved by the system at maximum substrate concentration. The Michaelis constant Km is the substrate concentration at which the reaction rate is half of Vmax.
a. Plot the data given below with [S] on the x axis.
| [S] (M) | V (M/min) |
| 10.00 | 0.762577 |
| 13.33 | 1.147317 |
| 23.33 | 1.571784 |
| 50 | 2.300816 |
| 100 | 3.20466 |
| 150 | 3.58439 |
| 200 | 3.692158 |
| 250 | 3.992428 |
What are the optimized values for Vmax and Km
b. It is usually more convenient to arrange the equation in linear form by using the double reciprocal plot as illustrated by the following equation,
1/V = Km/Vmax 1/[S]+1/Vmax
Plot the reciprocals (1/[S] vs. 1/V) and fit the data to a straight line using LINEST. Plot the equation on the graph. What are the values Vmax of Km and using this method?
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