Question: my variable being tested is only NaCl concentration on lactase activity. I plan on getting a final total solution of 10ml Design an experiment that

 my variable being tested is only NaCl concentration on lactase activity.my variable being tested is only NaCl concentration on lactase activity. I plan on getting a final total solution of 10ml
I plan on getting a final total solution of 10ml Design an
experiment that will test your variable, you will need: lactase, lactose, a

Design an experiment that will test your variable, you will need: lactase, lactose, a buffer, and potentially the variable you are testing (if it is something like NaCl concentration). You need to use the C1V1=C2V2 equation to determine the amount lactose, buffer, etc in your solution-look for the parameters in the lab manual. M What is available? 6% Lactose solution Goat Milk Cow Milk Lactase enzyme 1 M SPG buffer pH 4 and pH 10 water baths 0,20, 40, 60, 80 C 5M NaCl Dish soap deionized water Sucrose What do you want in your final solutions? s 2 % lactose 0.1-0.2 M SPG buffer- for a consistent pH 5-15 ml total volume Design an experiment that will test your variable, you will need: lactase, lactose, a buffer, and potentially the variable you are testing (if it is something like NaCl concentration). You need to use the C1V1=C2V2 equation to determine the amount lactose, buffer, etc in your solution-look for the parameters in the lab manual. M What is available? 6% Lactose solution Goat Milk Cow Milk Lactase enzyme 1 M SPG buffer pH 4 and pH 10 water baths 0,20, 40, 60, 80 C 5M NaCl Dish soap deionized water Sucrose What do you want in your final solutions? s 2 % lactose 0.1-0.2 M SPG buffer- for a consistent pH 5-15 ml total volume

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