Nicotiana tabacum cells are cultured to high density for production of polysaccharide gum. The reactor used is
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Nicotiana tabacum cells are cultured to high density for production of polysaccharide gum. The reactor used is a stirred tank, containing initially 100 liters of medium. The maximum specific growth rate of the culture is 0.18 d-1 and the yield of biomass from substrate is 0.5 g/g. The concentration of growth-limiting substrate in the medium is 3% (w/v). The reactor is inoculated with 1.5 g/l cells and operated in batch until the substrate is virtually exhausted.
Estimate the batch culture time and final biomass concentration.
Related Book For
Molecular Cell Biology
ISBN: 978-1429234139
7th edition
Authors: Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger, Anthony Bretscher, Hidde Ploegh, Angelika Amon, Matthew P. Scott
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