Question: Peptides can be separated using an ion-exchange column based on their isoelectric (pl) values. At which pH values would two diferent peptides, one with a
Peptides can be separated using an ion-exchange column based on their isoelectric (pl) values. At which pH values would two diferent peptides, one with a pl of 5.9 and the other with a pl of 8.7, bind to a cation- and anion-exchange column? Each peptide may be capable of binding to each column at more than one pH value. anion-exchange column at cation-exchange column at pH=3.1 pHH=3.1 pH=6.5 pH=6.5 pH=11.0 pH=11.0
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