Phosphoric acid is a tri-protic acid, which can dissociate three different protons at three different pka's. These pka values are 2.2, 7.2 and 12.7. The graph below shows the titration of phosphoric acid with a strong base. I 14 Hd 12- 10 8 6 4- 2- 0 First midpoint [H,PO]= [HPO pk,= 2.2 0 0.5 Second midpoint [HPO] = [HPO] pk, 7.2. = Figure 2-1r Principles of Biochemistry, Ale 2006 Pearson Prentice Hall,Inc. 1.0 Third midpoint [HPO]=[PO] pk = 12.7 First endpoint 1.5 Equivalents of OH 2.0 Third endpoint Second endpoint 2.5 3.0 c) Provide the chemical equilibrium that describes the phosphoric buffer system that exists at pH 8.1. Clearly indicate the weak acid and it's conjugate base, and the names of the chemical compounds. (1 mark) d) Calculate to 1 decimal place, the ratio of base/acid at pH 8.1. Show all details of your working out. In each step of your working out use the chemical formulas of the compounds that constitute the weak acid and conjugate base. State the final answer in a complete sentence. (1 mark) I= e) Calculate to 2 decimal places the concentration of the conjugate acid and conjugate base for a total buffer concentration of 150 mM at pH 8.1. Show all details of your working out; in each step of your working out use the chemical formulas of the compounds that constitute the weak acid and conjugate base. Show all units and state the final answer in a complete sentence. (1 mark)