Please help on how to draw the cube plot. And show how table 2 is on the table, please. Also show the calculation of main and interaction effect.

Garotenoids are sommerciall important natyral pigments that are ysed as food colorants and sources of pigmentation of fish in aquaculture. Several microorganisms, such as the yeast Rhodotorula glutinis are able to produce carotenoids from low-cost substrates such as whey ultrafiltrate, sugar cane juice, corn starch hydrolysate, peat hydrolysate, grape must and sugar cane molasses. The growth medium usually also includes urea, KH2PO4 and NaCl. a) For a carotenoid production process development study using Design of Experiments methods, indicate whether the each item in the following list is a putative factor, objective or an outcome: Table 1. The list of factors, outcomes and objectives b) From DoE point of view, design 2 levels factorial experiment by choosing the factors and outcomes from the above list. Determine low (-) and high (+) levels of factors (by giving symbols for each in Table 2). Assume that you run the experiments and obtained results for each run and draw cube plot (you can also give symbols for the outcomes). Based on cube plot, calculate the main and interaction effects