pls help with calculation

*Start here: 3. Dilute the homogenate (1.0mL) with the 0.05M ascorbic acid buffer, pH4.5, by slowly adding in 4.0ml of ascorbic acid buffer in a test tube. Use the p1000 micropipette for measurement and pipette up/down to mix. 4. Heat the homogenate at 65C in a water bath for 4 minutes (Teams 1 and 5 ), 6 minutes (Teams 3 and 6 ), and 8 minutes (Teams 2 and 4). Mix frequently by swirling. 5. Transfer the homogenate to 4 microcentrifuge tubes (1.7mL) with 1.25mL in each. Be careful. You need not transfer the entire solid that precipitated. However, estimate the size of solid left (pea size, quarter size??) and note it in your lab notebook. Be sure to label your tubes with your team number. 6. Cool the sample on ice. Samples must be cold before proceeding to the next step. 7. Record the number of the position in the rotor where your tube is placed. Centrifuge the tubes at 10,000Xg for 20 minutes. During this time, prepare the calculation in step 9 and calibrate pH meters. 9. Calculate the volume of phosphate buffer to add for a 1:10 dilution ( 500mM to 50mM final) for the next step using all of your supernatant +X as your final volume. Check your math with the instructor/TA before continuing. Record your full calculation in your lab notebook. 10. Add mL of 0.5M sodium phosphate buffer, pH7 at 1:10 to your supernatant and let the solutions mix (using inversion) for 15 minutes on ice