Predict what each of the following experimental changes will do to the initial rate of dopa oxidation catalyzed by tyrosinase. Answer each with “increase”, “decrease”, or “no change.”

a.Increase the temperature of the reaction mixture from 25 C to 37 C.

b.Change the concentration of dopa from a value equal to 1 K _M to a value equal to 2 K _M .

c.Add a few drops of a 1 x 10 ^-3 M cysteine solution.

d.Increase the reaction temperature from 37 C to 100 C.

e.Decrease the pH of the reaction mixture from pH 7.0 to pH 1.0.

f.Change the concentration of tyrosinase from 1 g/assay to 2 g/assay.