Problem 1 (4 points). (Anisotropy decay). A spherical protein molecule of 23,000MW and fluorescence lifetime of 4 ns is free to rotate in water at a temperature of 25 C. The time zero anisotropy for this protein at the wavelength of excitation is ro=0.37. Calculate the value of the anisotropy at 2 ns after excitation assuming that the excitation is vertically polarized and the emission is observed through polarizers in the vertical and horizontal direction. The excitation is considered to be instantaneous. Problem 2 (4 points). (Anisotropy steady state) For the same molecule than in problem 1 calculate the value of the steady-state anisotropy