Problem Set # 3 BIOT 5631"Cell Culture Process for Recombinant Protein Production" Using the data in the excel spreadsheet provided, calculate: (a) cell specific growth rate (b) doubling time (c) cell specific glucose consumption rate (d) lactate production rate (e) cell specific IgG production (antibody) rate Calculate these parameters using both differential and integral methods. Hint 1: The values obtained by either differential or integral method should be similar to each other. Otherwise, one of the methods is incorrect. Hint 2: Use the following table to help you calculate the above rates. Hint 3: For all calculations except IgG antibody rate focus only on the exponential phase of the growth. To calculate IgG antibody rate, perform the analysis for the whole culture period.

ZOOM Days in Culture Viable cell density Viability Nova pH Nova Glucose (Gluc) g/L Nova Lactate (Lac) g/L Titer (mg/L) 0.0 1.50E+05 90.3 7.201 6.1 0 1.1 3.09E+05 95.2 7.042 6 0.05 18.05 1.9 5.12E+05 93.9 7.074 5.8 0.1 29.55 2.2 6.40E+05 93.8 7.064 5.5 0.3 36.65 2.7 9.20E+05 93.6 6.916 5.2 0.5 58.5 3.2 1.38E+06 93.6 6.639 4.75 0.8 65 3.7 1.92E+06 88.8 6.688 4.1 1.1 70.5 4.1 3.01E+06 92.4 6.698 3.1 1.8 135.5 4.6 3.95E+06 93.2 6.683 2 2.8 189.5 5.0 5.36E+06 92.1 6.666 0.5 3.89 250.5 5.6 6.47E+06 91.6 6.692 4.1 368.5 6.0 7.09E+06 88.7 6.743 4.05 495 6.6 8.16E+06 87.7 6.73 4 641.5 7.0 9.36E+06 87 6.75 3.8 767.6 7.7 9.84E+06 84.6 6.885 3.76 962.5 8.0 9.92E+06 81.8 6.81 3.7 1148 8.6 1.08E+07 79.1 6.851 ooo ooooooooo 3.44 1366 9.0 1.05E+07 76.1 6.851 3.4 1575 9.6 1.02E+07 72.7 6.964 3.2 1756 10.0 9.36E+06 63.7 6.919 3.4 1962 10.5 7.87E+06 59.2 6.961 3.2 2112 10.9 7.10E+06 47.9 6.985 3.12 2215 11.5 5.72E+06 40.8 7.044 3 2328