Question: Put into steps for a lab : Max was a patient who had a serious bacterial infection and samples were taken from him. The appearance
Put into steps for a lab :
Max was a patient who had a serious bacterial infection and samples were taken from him. The appearance of black colonies on the Salmonella Shigella agar allowed for the identification of the bacteria isolated from Max's blood as Salmonella. The chicken shed, the source of the meat that Max ate, was also sampled for feces. In the lab, a wire loop was rendered inert by burning it with a Bunsen burner; subsequently, it was cooled by touching a sterile agar plate. We streaked the wire loop across the Salmonella Shigella agar plate after dipping it into the fecal sample. To re-sterilize the wire loop, it was placed over the flame of the Bunsen burner. After that, the agar plate was covered and left in an incubator overnight. We used a T-streak approach to isolate Salmonella colonies. The inoculating loop was used to streak the fecal samples onto half of a sterile agar plate.
All Rights ReservedIt was then repeated once more to cover the remaining quadrant, this time streaking the germs towards the next quadrant using a sterile loop. Lastly, a sterile loop was used to pick an isolated Salmonella colony, which was then placed on a
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