Question: Q 2 : A chromatogram of a two - component mixture on a 2 5 c m - packed liquid chromatography column is shown below,

Q2: A chromatogram of a two-component mixture on a 25cm-packed liquid chromatography column is shown below, The flow rate was 0.4mlmin
a) Find the retention times for A and B.
b) Calculate the relative retention ( selectivity ) for A, B
c) Calculate the capacity factor K for A and B
d) Find the average number of plates for the column
e) Find the average plate height
f) Calculate the resolution (Rs) for A,B
Rs=2[(tR)B-(tR)A]WA+WB
 Q2: A chromatogram of a two-component mixture on a 25cm-packed liquid

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