Question: Question 1. You are running reverse phase HPLC on two compounds (compound A and compound B). The mobile phase is 8:1 ammonium formate buffer:acetonitrile (pH
Question 1.
You are running reverse phase HPLC on two compounds (compound A and compound B). The mobile phase is 8:1 ammonium formate buffer:acetonitrile (pH = 4.5). Both compounds are chemically neutral. Compound A has a logP of 1.0 whereas compound B has a logP of 3.2. With the information provided which of the following would you predict to be true?
Question 1 options:
| A) | Compound A will elute before Compound B. |
| B) | Compound B will elute before Compound A. |
| C) | It is not possible to say with the information given. |
| D) | Compound A and B will elute at the same time. |
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