Question: Question 2 1 - 2 8 Explain to me each question and what E Coli is for complementation and what E Coli is for combination?

Question

21 - 28

Explain to me each question and what E Coli is for complementation and what E Coli is for combination?

Questions

21 - 28

: There are five rII mutants. Mutants

1

and

2

are two non

-

overlapping points mutations in rIIA. Mutants

3

and

4

are two overlapping deletion mutations in

rIIB. Mutant

5

is a deletion that overlaps mutants

1

and

3

but does not overlap mutants

2

and

4 .

21 0 / 4

points

Mutant

5

will not be able to complement with all other

4

mutants.

True

O False

Correct Answer: True

| 4 / 4

points

Mutant

3

will be able to recombine with two of the other four mutants and produce wild

-

type recombinants, and mutant

4

will be able to recombine with three of the other

four mutants and produce wild

-

type recombinants

True

False

/ 4 / 4

points

You perform a high m

.

.

i infection of mutants

1

and

2

on E

.

coli strain B

.

Then you harvest the lysate and perform a low m

.

.

i infection on E

.

coli strain K

.

Finally, you dilute this

second lysate and perform low m

.

.

i infections on separate plates with E

.

coli B and E

.

coli K

.

plaques; select "Can't tell" if the information provided is insufficient to predict the result with certainty.

More on E

.

coli B

More on E

.

coli K

O Equal number

No plaques

Can't tell

| / 4

points

You perform a high m

.

.

i infection of mutants

3

and

4

on E

.

coli strain B

.

Then you harvest the lysate and perform a low m

.

.

i infection on E

.

coli strain K

.

Finally, you dilute this

second lysate and perform low m

.

.

i infections on separate plates with E

.

coli B and E

.

coli K

.

plaques; select "Can't tell" if the information provided is insufficient to predict the result with certainty.

More on E

.

coli B

More on E

.

coli K

Equal number

O No plaques

Can't tell

4 / 4

points

You perform a high m

.

.

i infection of mutants

3

and

4

on E

.

coli strain B

.

Then you harvest the lysate and perform a low m

.

.

i infection on E

.

coli strain K

.

Finally, you dilute this

second lysate and perform low m

.

.

i infections on separate E

.

coli B and E

.

coli K plates.

Predict the result. Select "Equal Number" if the E

.

coli B and E

.

coli K plates will have some plaques in equal proportions; select

"

No Plaques" if both plates will show

0

plaques; select "Can't tell" if the information provided is insufficient to predict the result with certainty.

More on E

.

coli B

More on E

.

coli K

Equal number

O No plaques

Can't tell

You perform a low m

.

.

i infection of mutants

3

and

4

on E

.

coli strain B

.

Then you harvest the lysate and perform a high m

.

.

i infection on E

.

coli strain K

.

Finally, you dilute this

second lysate and perform low m

.

.

i infections on separate E

.

coli B and E

.

coli K plates

Predict the result. Select "Equal Number" if the E

.

coli B and E

.

coli K plates will have some plaques in equal proportions; select

"

No Plaques" if both plates will show

0

More on E

.

coli B

More on E

.

coliK

Equal number

No plaques

Can't tell

4 / 4

points

You perform a high m

.

.

i infection of mutants

4

and

5

on E

.

coli strain B

.

Then you harvest the lysate and perform a high m

.

.

i infection on E

.

coli strain K

.

Finally, you dilute

this second lysate and perform low m

.

.

i infections on separate E

.

coli B and E

.

coli K plates.

Predict the result. Select "Equal Number" if the E

.

coli B and E

.

coli K plates will have some plaques in equal proportions; select

"

No Plaques" if both plates will show

0

plaques; select "Can't tell" if the information provided is insufficient to predict the result with certainty.

More on E

.

coli B

More on E

.

coliK

Equal number

No plaques

Can't tell

| 4 / 4

You perform a high m

.

.

i infection of mutants

1

and

3

on E

.

coli strain K

.

Then you harvest the lysate and perform a high m

.

.

i infection on E

.

coli strain B

.

Finally, you dilute

this second lysate and perform low m

.

.

i infections on separate E

.

coli B and E

.

coli K plates.

Predict the result. Select "Equal Number" if the E

.

coli B and E

.

coli K plates will have some plaques in equal proportions; select

"

No Plaques" if both plates will show

0

plaques; select "Can't tell" if the information provided is insufficient to

plaques; select "Can't tell" if the information provided is insufficient to predict the result with certainty.

More on E

.

coli B

More on E

.

coli K

Equal number

No plaques

Can't tell

Question 2 1 - 2 8 Explain to me each question

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