REAGENTS AND MATERIALS REACTION SETUP Clean your two 10mL graduated pipets (from kit) by rinsing with. (Step 2b) Label one pipet and the other (Step 2b) Rinse each pipet times with mL of the appropriate reagent (Steps 2c \& d) Make sure to times so that the solution coats the inner walls of the pipet FINDING THE WAVELENGTH OF MAXIMUM ABSORBANCE Blank the spectrophotometer using a cuvette filled with Rinse the cuvette times with (Step 4) _(Step 3) Fill cuvette with (Step 4) Run a full spectrum measurement and select maximum peak (Step 4) Record wavelength of maximum absorbance: REACT CRYSTAL VIOLET WITH NAOH AND RECORD ABSORBANCE OVER TIME Use the CV pipet to transfer mL of crystal violet to the CV beaker. (Step 6a) Use the NaOH pipet to transfer mL of sodium hydroxide to the NaOH beaker (Step 6b) (Step 6c) Mix solutions by Rinse cuvette times with small portions of (Step 6d) I will mix the solutions, rinse the cuvette, fill the cuvette, and start the measurement within Take measurement (Step 6d) seconds. (Gray box) Empty cuvette and immediately rinse cuvette times with (Step 6d) Analyze the data. (Details in Steps 7a-e) Repeat this box for Trials 2 and 3