Question: Some possibly useful equations/ constants; AGO = -RTInk., AG = AG + RTInQ AG = AH - TAS S = RInW R = 1.9872 cal

Some possibly useful equations/ constants; AGO = -RTInk., AG = AG + RTInQ AG = AH - TAS S = RInW R = 1.9872 cal K' mol, RT= 0.592 at 298K Vmax [S] [Eltot*Kcat*[S] Vo = Vo = KM+[S] KM+[S] -AGOT -AAGOF k = KBT Kcat e RT e RT h Kuncat KET = 6.24 x 10 - at 298K = Vmax KM,app = KM * OC h Vmax,app5 points: A piece of DNA with the sequence 5' - AGGTCATGCGGATACGA-3' is subjected to dideoxy terminator sequencing and resolved on a gel. Please give an expected gel result from this sequencing run. A GC D(5 points) Draw the base pair between a G and C residues in double stranded DNA (B form) Base pair between G an C Guanine Cytosine O NH2 H-- H H2N H- -- N O N H- - - - -0 H N 1 NH2 C

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