Step 1 of the protocol is preparing the Extraction Buffer. Calculate how to prepare 10 mM Tris (pH 7.4) containing 1 mM 2-mercaptoethanol, 1 mM PMSF, 1 mM EDTA.

*note* For this assignment, answer these questions as if you were making 200 mL. (enough for 2 people or if you want to prepare twice as much tissue)

After preparing 200 mL of 10 mM Tris, you will add the following reagents: [ Select ] mL of 0.2 M 2-Mercaptoethanol stock solution (final concentration = 1 mM 2-Mercaptoethanol [ Select ] mL of 0.1 M PMSF stock solution (final concentration = 1 mM PMSF) [ Select ] mL of 0.5 M EDTA stock solution (final concentration = 1 mM ETDA)