Question: Table 1 presents a data for an enzymatic reaction. a) Which inhibitor binds with higher affinity to the free enzyme? Justify your answer. (4 marks)

Table 1 presents a data for an enzymatic reaction. a) Which

Table 1 presents a data for an enzymatic reaction. a) Which inhibitor binds with higher affinity to the free enzyme? Justify your answer. (4 marks) b) If the same concentration of inhibitor were used in each experiment, which inhibitor would give the smallest value of KM,app ? Justify your answer (4 marks) c) If the value for KM is 1M, what is the ratio of KL/KM for each inhibitor? How is this related to the competing equilibria for binding of the substrate vs. the inhibitor to the enzyme? (7 marks)

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