Question: The eluate from a high-performance liquid chromatography (HPLC) separation was analyzed using electrospray time-of-flight mass spectrometry (TOF-MS). The molecular ions of two peptides, each with

The eluate from a high-performance liquid chromatography (HPLC) separation was analyzed

The eluate from a high-performance liquid chromatography (HPLC) separation was analyzed using electrospray time-of-flight mass spectrometry (TOF-MS). The molecular ions of two peptides, each with a mass of 750.2Da, are separated with a 10% valley between them. The molecular ion peaks have a width at half-height of 0.0310 Da and differ in mass by 0.0682 Da. Calculate the resolving power of the TOF-MS using the 10% valley formula. resolving power: Calculate the resolving power of the TOF-MS using the half-width formula. resolving power

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock blur-text-image

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Students Have Also Explored These Related Chemical Engineering Questions!

The following histograms show the number of years in office for Democratic and Republican U.S. senators. a. Describe the shape of each histogram. b. Because of the shapes, what measures of center...

Protein separation techniques 1 When separating proteins on SDS - 2 points PAGE, all proteins are positively charged because they are treated with Sodium dodecyl sulphate True False Clear selection 2...

A 99Mo-99mTc generator is eluted Monday at 7:00 AM, producing 1.8 Ci of 99mTc in the eluate vial, in 20 ml saline. What volume of eluate should be withdrawn from the eluate vial into a syringe in...

Two proteins A and B are chromatographed through a bed. The total volume of the bed is 100 L and void fraction is 0.30. The equilibrium constants K are 10.0 and 12. 0 for A and B, respectively....

You decide to perform ion exchange chromatography next. Howevec, you do not know the isoelectric point (p) of the protein. You decide to perform isoelectric focuting. This is an anaytical technique -...

REQUIRED Before lab, create a flow diagram for today's experiment below. Your flow diagram should include the following samples: filtered dialysate, Wash 1 , Wash 2 , Eluate 1 and Eluate 2 . List the...

database Feypone time is the time it takes from when a tohd or wite recacet is loniod in mhicis den mertit. 4= In the Parti: 1. Response time in the time it thies from when a read of write roquent is...

I just need Q3 Step 2 is : Understand Client environment and controls Alpine Cupcakes, Inc. Audit Risk Assessment Memo Audit Year December 31, 20X2 Garcia and Foster, CPAS Performed by: SDM Reviewed...

Reagent grade vanadyl sulphate supplied commercially contains sulphuric acid and water as impurities. In an experiment the purity of a sample of vanadyl sulphate was measured using ion exchange...

answer 1 -8 18. Whistler Township Volunteer Fire Department (WTVFD) because they feel good about giving back to their com Nolan Fenwick raced out the front door of the Target view it as a hobby, and...

Decision Analysis - Luxury Car vs. Sportscar The next 15 questions refer to the following scenario: A car manufacturer can launch either a new luxury sedan (L) or a new high-performance sports car...

On-the-job experience typically enhances a persons productivity in that particular job. If the persons salary increases to reflect increased experience but the additional experience has no relevance...

Is it possible to produce an iron-carbon alloy that has a minimum tensile strength of 690 MPa (100,000 psi) and a minimum ductility of 40%RA? If so, what will be its composition and microstructure...

Problem 1 Recreate the cash flow spreadsheet provided in Table 6 . 6 and reproduce the results. a ) In what year do you first see a positive cash flow? b ) What is the present value of the cash flow...

Write Python code to complete the following problems in a single . py file. Problems: Generate and print the truth table for the logical expression ( A AND B ) OR ( NOT C ) . Define four sets with...

Which part of the data analysis cycle needs more attention?

Are there new or different data points that need to be considered?

How will staff be engaged in this process?