The initial rate at which it was emitted O₂ As a result of an enzymatic reaction with a substrate S₁ was measured at different initial concentrations of the substrate and the following results were obtained

A. Consider the constant Michaelis Mentan K_m and the maximum speed V_max

B. In another series of experiments, a non-competitive inhibitor was added to the system so that the maximum reaction speed dropped to 75% of the value when there is no inhibitor. We will mark [S] The concentration of the substrate that gives a reaction speed that is half of the maximal speed without an inhibitor. How many times should you increase the [S] When there is a non-competitive inhibitor in the system in order to get the same response speed?

C. The same enzyme also catalyzes the reaction of another substrate S₂ leading to creation CO₂

Get an expression for the relationship between the speed of formation O₂ and speed of formation CO₂ As a function of the enzyme concentrations, the two substrates and the rate constants of the catalysis mechanisms.

\begin{tabular}{|c|c|c|c|c|c|} \hline[S1]/M & 0.05 & 0.017 & 0.01 & 0.005 & 0.003 \\ \hline v/mm3min1 & 16.6 & 12.4 & 10.1 & 6.6 & 3.3 \\ \hline \end{tabular} E+S1k1k1ES1k2P1+O2+EE+S2k~1k~1ES2k~2P2+CO2+E