Question: There are two unknown samples analyzed by HPLC: Unknown 1 and Unknown 2. Calculate the number of theoretical plates (N, in plates/column) and plate height

There are two unknown samples analyzed by HPLC: Unknown 1 and Unknown 2. Calculate the number of theoretical plates (N, in plates/column) and plate height (H, in mm/plate) for the latest peak (Peak 2) in each unknown.

Unknown 1 Peak 2 tR: 5.212 mins.

Unknown 1 Peak 2 sigma: 0.2554 mins.

Unknown 2 Peak 2 tR: 5.256 mins.

Unknown 2 Peak 2 sigma: 0.2273 mins.

The flow rate is 1 mL/min.

Unknown 1 H =

Unknown 1 N =

Unknown 2 H =

Unknown 2 N =

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