This problem will take you through the steps for determining the total AAT (IU) in a fraction starting from the chart tracing data. The assays are performed as described in the coursepack for Unit E: the final volume of the assay is 3.00 mL and 0.100 mL of the diluted fraction is used in the assay. Report your answers rounded to 3 significant figures and include units unless otherwise indicated. The assay measures the oxidation of NADH ( 340 nm = 6220 M-1cm-1) over time. As such, the absorbance will decrease as the reaction proceeds, but for the purpose of calculating enzyme activity, the absolute value of absorbance change is used. Therefore, when the question refers to the "chart squares per minute" of change, it refers to the absolute value of the "chart squares per minute". You have set the chart recorder to 1.10 absorbance units full scale (100 squares) and have diluted your fraction 190-fold. After performing the assay, you estimate the slope to be 5.70 chart squares per minute.

What is the concentration change in mM/min?

This problem will take you through the steps for determining the total AAT (IU) in a fraction starting from the chart tracing data. The assays are performed as described in the coursepack for Unit E: the final volume of the assay is 3.00 mL and 0.100 mL of the diluted fraction is used in the assay. Report your answers rounded to 3 significant figures and include units unless otherwise indicated. The assay measures the oxidation of NADH (C340 mm = 6220 M-1cm1) over time. As such, the absorbance will decrease as the reaction proceeds, but for the purpose of calculating enzyme activity, the absolute value of absorbance change is used. Therefore, when the question refers to the "chart squares per minute of change, it refers to the absolute value of the chart squares per minute". You have set the chart recorder to 1.10 absorbance units full scale (100 squares) and have diluted your fraction 190-fold. After performing the assay, you estimate the slope to be 5.70 chart squares per minute. What is the absorbance change per minute? (4 pts.) 0.0627 A/min You are correct. Your receipt no. is 160-1892 Previous Tries What is the concentration change in mM/min? (4 pts.) Submit Answer Tries 0/5 How many International Units (umol/min) of enzyme are in the assay? (4 pts.) Subrrit Answer Tries 0/5 What is the concentration of AAT (umol/min/mL) in the undiluted fraction? (4 pts.) Submit Answer Tries 0/5 If the volume of the fraction 40.0 mL, what is the total amount of AAT in the fraction (umol/min)? (4 pts.) Subrrit Answer Tries 0/5 This problem will take you through the steps for determining the total AAT (IU) in a fraction starting from the chart tracing data. The assays are performed as described in the coursepack for Unit E: the final volume of the assay is 3.00 mL and 0.100 mL of the diluted fraction is used in the assay. Report your answers rounded to 3 significant figures and include units unless otherwise indicated. The assay measures the oxidation of NADH (C340 mm = 6220 M-1cm1) over time. As such, the absorbance will decrease as the reaction proceeds, but for the purpose of calculating enzyme activity, the absolute value of absorbance change is used. Therefore, when the question refers to the "chart squares per minute of change, it refers to the absolute value of the chart squares per minute". You have set the chart recorder to 1.10 absorbance units full scale (100 squares) and have diluted your fraction 190-fold. After performing the assay, you estimate the slope to be 5.70 chart squares per minute. What is the absorbance change per minute? (4 pts.) 0.0627 A/min You are correct. Your receipt no. is 160-1892 Previous Tries What is the concentration change in mM/min? (4 pts.) Submit Answer Tries 0/5 How many International Units (umol/min) of enzyme are in the assay? (4 pts.) Subrrit Answer Tries 0/5 What is the concentration of AAT (umol/min/mL) in the undiluted fraction? (4 pts.) Submit Answer Tries 0/5 If the volume of the fraction 40.0 mL, what is the total amount of AAT in the fraction (umol/min)? (4 pts.) Subrrit Answer Tries 0/5