This problem will take you through the steps for determining the total AAT (IU) in a fraction starting from the chart tracing data. The assays are performed as described in the coursepack for Unit E; the final volume of the assay is 3.00mL and 0.100mL of the diluted fraction is used in the assay. Report your answers rounded to 3 significant figures and include units unless otherwise indicated. calculating enzyme activity, the absolute value of absorbance change is used. Therefore, when the question refers to the "chart squares per minute" of change, it refers to the absolute value of the "chart squares per minute". You have set the chart recorder to 1.10 absorbance units fuil scale (100 squares) and have diluted your fraction 140 -fold. After performing the assay, you estimate the slope to be 7.60 chart squares per minute. What is the absorbance change per minute (A/min)?(4 pts.) Tries o/s What is the concentration change in mM/min? (4 pts.) Tries 0/5 How many International Units (umol/min) of enryme are in the assay? (4 pts.) Tries ors What is the concentration of AAY (umol/min/mi) in the undiluted fraction? (4 pts.) Tres 0/5 If the volume of the fraction is 46.0mL, what is the total amount of AAT in the fraction (umolmin)? (4 pts.) Tires o/s