Question: To use CRISPR technology to target two different genomic sequence sites at the same time in the same cell, you would need to: have two

 To use CRISPR technology to target two different genomic sequence sites
at the same time in the same cell, you would need to:

To use CRISPR technology to target two different genomic sequence sites at the same time in the same cell, you would need to: have two tracrRNAs, present specific for each site have two crRNAs, present specific for each site use cell cycle blockers use Cpf 1 for one site and Cas9 for the other Which of the following is NOT true of Cas9: It makes a blunt-ended double stranded break it requires a crRNA and tracrRNA a it has two separate nuclease domains it cuts a TTN PAM

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