Tris, a base, is a popular buffer for biochemical research. Its pKa is strongly dependent on temperature. Would it make a very good buffer for a scanning calorimetry experiment? Why or why not? Assuming that Ka 8.3 109 M, calculate the ratio of acid to base at pH 8.0. Let the total concentration of Tris be 150 mM, and divide the stock into to two parts. To one, add 10 mM HCl. How does the pH change? Hint: assume complete dissociation of HCl. To the other, reduce the concentration to 30 mM. How does the pH change?