Question: Using the Van Deemter equation, HETP, and N, as appropriate, explain how the following changes may affect the efficiency of separation in chromatography also talk

  1. Using the Van Deemter equation, HETP, and N, as appropriate, explain how the following changes may affect the efficiency of separation in chromatography also talk about the plates.

    a) Increase the flow rate of nitrogen from 10 cm/sec to 40 cm/sec b) Increase the length of the column from 30m to 60m c) Based on the chart below, which carrier gas will you use, why?

Using the Van Deemter equation, HETP, and N, as appropriate, explain

N, 1.2 HEPT (mm) 0.8 11 He !1 H2 0.4 20 40 60 80 carrier gas velocity (cm/sec)

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