Question: When using CRISPR-Cas9 for gene editing, two primary repair mechanisms can be leveraged: homology-directed repair (HDR) and non-homologous end joining (NHEJ). Identify the statements that
When using CRISPR-Cas9 for gene editing, two primary repair mechanisms can be leveraged: homology-directed repair (HDR) and non-homologous end joining (NHEJ). Identify the statements that are true regarding these mechanisms. ONHEJ is leveraged for targeted insertions of large DNA sequences. Both HDR and NHEJ are naturally occurring DNA repair mechanisms harnessed in CRISPR-Cas9 technology. NHEJ can introduce insertions or deletions (indels) at the DNA break site. HDR requires a template DNA strand to guide the repair. HDR is the only repair mechanism used in humans because it repairs perfectly while end-joining always introduces mutations
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