Question: When you are performing pipette (column) chromatography, how do you know when the compound you are collecting has finished eluting off the column? Observing the

When you are performing pipette (column) chromatography, how do you know when the compound you are collecting has finished eluting off the column? Observing the first spot on your TLC plate means that you can stop collecting fractions as your compound has eluted You will be able to visually observe the compound eluting off your column in all cases TLC analysis will indicate when fractions contain no more of the desired compound and it has eluted off the column You can stop collecting after five fractions as this is standard protocol. Eluting more than five fractions will waste solvent

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock blur-text-image

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Students Have Also Explored These Related Chemistry Questions!

Discussion and Conclusion Prompt Questions Do not number the answers in your typed discussion section, simply cover these topics in a logical way. 1. Discuss the addition of calcium chloride drying...

i cant figure out what compounds each color band (light green, green, and yellow) are. Once I have those three compounds, I need their structures drawn out. Thank you Pre-lab assignment 1. On the...

Asking: Pick your four concentrations in the range of 10-75 M and calculate the dilutions youll need to perform from the stock solution to prepare them. This week in lab, we will be performing two...

PLEASE READ CAREFULLY THE CASE STUDY PROVIDED AND FEEL FREE TO ADD HERE YOUR COMMENTS FOR EXAMPLE LIKES DISLIKES WORDS OR PHRASES YOU DO NOT UNDERSTAND ANY COMMENTS THAT WILL IMPROVE THE DIALOGUE...

Introduction to Ridgeline Mountain Outfitters (RMO) Ridgeline Mountain Outfitters (RMO) is a large retail company that specializes in clothing and related accessories for all types of outdoor and...

please follow step by step with results, calculation and diagrams thanks Experiment 1. Potentiometric Titrations Learning outcomes - Operation and use of a pH electrode - Use of titration to...

4. Plastic Pipette. For his chemistry experiment, Gene uses a cylindrical plastic pipette of diameter 0.80 cm, which has a bulb at the end that can be squeezed to pick up water. The volume of the...

Table 8.1, page 249, gives the initial volumes of Bro,...and 5,0, for seven different kinetics runs you will conduct in this fab activity. Determine the initial concentrations of BIO-.1.H. and 5,0...

please help Data Sheet The Determination of Acid Content in Vinegar Reading assignment: Chang, Chemistry 10 edition, pages 153-156. We will use a titration to determine the concentration of acetic...

Table 1: mass (9) mass of Empty 250 ml beaker (mi) mass of 250 mL beaker + Unknown (m2) mass of Unknown (m2 - 1) = (ms) mass of Whatman filler No. 44 (m) mass of Whatman filter No. 44 +BaSO.(ms) mass...

Barry Egan is a currency speculator. Barry believes that the Japanese yen will fluctuate widely against the U.S. dollar in the coming month. Currently, one-month call options on Japanese yen () are...

While the year is not over, Z-ban Corporation is expecting net operating income for 2020 to be about $750,000. The corporation makes quarterly charitable contributions of $35,000, and has already...

Conclude findings of FINTECH research and provide recommendations with Harvard referencing format

undefined 44 years You borrow money on a self liquidating installment loan (equal payments at the end of each year, each payment is part principal part interest) Loan amount $133,000 Interest Rate...

=+output ratio is about 2.5. Suppose that the production function is CobbDouglas, so that the capital share in output is constant, and that the United States has been in a steady state. (For

=+1. Suppose an economy described by the Solow model has the following production function: Y = K1/2(LE) 1/2. a. For this economy, what is f(k)?

=+ How does this differ from the Solow model?