Xylose isomerase extracted from the thermophile Thermoanaerobacterium thermosulfurigenes is capable of using glucose and xylose as...
Fantastic news! We've Found the answer you've been seeking!
Question:
Transcribed Image Text:
Xylose isomerase extracted from the thermophile Thermoanaerobacterium thermosulfurigenes is capable of using glucose and xylose as substrates. Applying each substrate separately, the rates of reaction are measured at 60°C using an enzyme concentration of 0.06 mg ml. Initial Initial Reaction Reaction Velocity (Jumol ml min ) Glucose Velocity (umol ml min) Xylose Concentration Concentration (mM) (mM) 80 0.151 20 0.320 100 0.194 50 0.521 300 0.385 100 0.699 500 0.355 300 1.11 750 0.389 500 1.19 1000 0.433 700 1.33 1400 0.445 900 1.13 (a) What is the relative catalytic efficiency of the enzyme using xylose as substrate compared with glucose? (b) What conclusions can you draw about the substrate specificity of this enzyme? Xylose isomerase extracted from the thermophile Thermoanaerobacterium thermosulfurigenes is capable of using glucose and xylose as substrates. Applying each substrate separately, the rates of reaction are measured at 60°C using an enzyme concentration of 0.06 mg ml. Initial Initial Reaction Reaction Velocity (Jumol ml min ) Glucose Velocity (umol ml min) Xylose Concentration Concentration (mM) (mM) 80 0.151 20 0.320 100 0.194 50 0.521 300 0.385 100 0.699 500 0.355 300 1.11 750 0.389 500 1.19 1000 0.433 700 1.33 1400 0.445 900 1.13 (a) What is the relative catalytic efficiency of the enzyme using xylose as substrate compared with glucose? (b) What conclusions can you draw about the substrate specificity of this enzyme?
Expert Answer:
Answer rating: 100% (QA)
Ans Let the molar mass of xylose isomerase from Thermoanaerobacterium th... View the full answer
Related Book For
Probability & Statistics for Engineers & Scientists
ISBN: 978-0130415295
7th Edition
Authors: Ronald E. Walpole, Raymond H. Myers, Sharon L. Myers, Keying
Posted Date:
Students also viewed these accounting questions
-
Draw the pH-activity profile for an enzyme with one catalytic group at the active site. The catalytic group is a general-acid catalyst with a pKa of 5.6.
-
What conclusions do you draw from this about the statement at the start of the chapter that if we only draw upon one particular frame, then this will take us away from thinking about what is going on...
-
What overall conclusions do you draw about why Barrett made the changes he did? Which issues were dominant? Why?
-
Matheny Inc. went public 3 years ago. The board of directors will be meeting shortly after the end of the year to decide on a dividend policy. In the past, growth has been financed primarily through...
-
What is goodwill ? How does it arise? How much goodwill did TELUS report on December 31, 2011? How does TELUS amortize its goodwill ? How much new goodwill did TELUS add in 2011? Is it possible that...
-
Refreshing Salons adjusted trial balance follows. Prepare Refreshing Salons income statement for the year ended December 31, 2024. REFRESHING SALON Adjusted Trial Balance December 31, 2024 Account...
-
In a random sample of 600 cars making a right turn at a certain intersection, 157 pulled into the wrong lane. Test the null hypothesis that actually \(30 \%\) of all drivers make this mistake at the...
-
You are the audit senior of Ball Construction Corporation (BC), a small public company that enters into construction contracts with individuals and developers and builds to their specifications. BC...
-
In this group project, you are required to conduct a legal analysis and develop a "legal argument" WHICH is merely a legal analysis. The premises of a legal argument consist of facts, laws, your...
-
Open the start file EX2019-ChallengeYourself-6-3 . The file will be renamed automatically to include your name. If the workbook opens in Protected View, click the Enable Editing button in the Message...
-
How to format the launched_date and end_date columns to datetime format this is my code: campaign_df['launched_date'] = pd.to_datetime(campaign_df['launched_date'], format='%Y-%m-%d')...
-
Kobayashi Corporation reports in the current liability section of its statement of financial position at December 31, 2014 (its year-end), short-term obligations of 15,000,000, which includes the...
-
Jeonbuk Company has two temporary differences between its income tax expense and income taxes payable. The information is shown below. The income tax rate for all years is 40%. Instructions (a)...
-
Santana Company exchanged equipment used in its manufacturing operations plus R$2,000 in cash for similar equipment used in the operations of Delaware Company. The following information pertains to...
-
Talarczyk Company sold 10,000 Super-Spreaders on July 1, 2015, at a total price of 1,000,000, with a warranty guarantee that the product was free of any defects. The cost of the spreaders sold is...
-
Main Company sells 100 televisions on June 1, 2015, at a total price of 35,000 with a warranty guarantee that the product was free of any defects. The assurance warranties extend for a 2-year period...
-
Question Text: Consider the following line of Java code.'"Hello,World'" is which of the following? System.out.println("Hello, World!"); Select one: a. a class b. a method c. an object d. an operator...
-
Sue Deliveau opened a software consulting firm that immediately paid $2,000 for a computer. Was this event a transaction for the business?
-
The data in the following table represent the number of hours of relief provided by 5 different brands of headache tablets administered to 25 subjects experiencing fevers of 38?C or more. Perform...
-
According to Chemical Engineering an important property of fiber is its water absorbency. The average percent absorbency of 25 randomly selected pieces of cotton fiber was found to be 20 with a...
-
A paint-store chain produces and sells latex and semi gloss paint. Based on long-range sales, the probability that a customer will purchase latex paint is 0.75. Of those that purchase latex paint,...
-
Let \(X_{1}, X_{2}, \ldots, X_{5}\) be 5 independent random variables. Find the moment generating function \[M_{\sum X_{i}}(t)=E\left(e^{t\left(X_{1}+X_{2}+\cdots+X_{5} ight)} ight)\] of the sum when...
-
Let \(X_{1}, X_{2}\), and \(X_{3}\) be independent normal variables with \[\begin{array}{lll}E\left(X_{1} ight)=5 & \text { and } & \sigma_{1}^{2}=9 \\E\left(X_{2} ight)=-2 & \text { and } &...
-
Refer to Exercise 6.36. (a) Show that \(2 X_{1}-X_{2}-4 X_{3}-12\) has a normal distribution. (b) Find the mean and variance of the random variable in part (a). Data From Exercise 6.36 6.36 Let X1,...
Study smarter with the SolutionInn App