Question: You are cloning using the pUC 1 9 vector. After transforming E , coli cells and plating them in the presence of ampicilin, you realize
You are cloning using the pUC vector. After transforming E coli cells and plating them in the presence of ampicilin, you realize that you forgot to add XGal to your plate. What would you expect to observe and is it possible to visually identify bacteria that have taken up the plasmid with the DNA Insert?
There would be butue colorsics and white colonies on the plate. The bacteria shat have taken we the plasmid with DeIA insert can be identifued by their thiue celler.
All colonies on the platie mould be white. You would not be abie to Adentity which bacteria tock up the plasmid with DRA Ineevt.
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