Question: You are cloning using the pUC 1 9 vector. After transforming E . coli cells and plating them in the presence of ampicillin, you observe
You are cloning using the pUC vector. After transforming E coli cells and plating them in the presence of ampicillin, you observe only white colonies on your plate? Which of the following is a possible explanation for this observation? choose all that apply
a You forgot to digest the vector with the same restriction enzyme as your DNA insert
b You forgot to add XGal to your plate
c All the E coli cells took up plasmid containing the DNA insert
d All the E coli cells took up plasmid that did not contain the DNA insert
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