Question: You just observed your pour plates from the loop dilution method after 48 hrs of growth at 37 degrees C. There is no growth

You just observed your pour plates from the loop dilution method after 

You just observed your pour plates from the loop dilution method after 48 hrs of growth at 37 degrees C. There is no growth on plates 2 and 3 (2nd and 3rd dilutions). Please explain at least two errors in technique that could have caused this result.

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