In the experiment of Figure 7.1, a met bio thr+ leu+ thi+ cell could become met+ bio+
Question:
In the experiment of Figure 7.1, a met− bio− thr+ leu+ thi+ cell could become met+ bio+ thr+ leu+ thi+ by a (rare) double mutation that converts the met− bio− genes into met+ bio+. Likewise, a met+ bio+ thr− leu− thi− cell could become met+ bio+ thr+ leu+ thi+ by three mutations that convert the thr− leu− thi− genes into thr+ leu+ thi+. From the results of Figure 7.1, how do you know that the occurrence of 10 met+ bio+ thr+ leu+ thi+ colonies is not due to these rare double or triple mutations?
Figure 7.1
Transcribed Image Text:
mat-blo-thr lou* thr 10 calls Mbed together met-bio-thr* lou thi and mat bio thr lou-thi- 10 calls mot' bio thr-lou-thi- 10 cells Nutrient agar plates lacking amino acids, blotin, and thiamine No colonies Bacterial colonies No colonies
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