Carotenoids are pigments responsible for much of the red we see in nature. For example, the bright

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Carotenoids are pigments responsible for much of the red we see in nature. For example, the bright red beak color of the male zebra finch is derived from carotenoids. Carotenoids are also important as antioxidants for humans and other animals, leading researchers to predict that carotenoids in birds may affect immune system function. To test this, a group of zebra finches was randomly divided into two groups (McGraw and Ardia 2003).

Ten individual finches received supplemental carotenoids in their diet, and 10 individuals did not. All 20 birds were then measured using an assay that measures cell-mediated immunocompetence (PHA) as well as an assay that measures humoral immunity (SRBC). The data are given below. The researchers had independent reasons to believe that neither assay score would be normally distributed, so they preferred a nonparametric test.

a. Does PHA differ on average between the birds that received supplemental carotenoids and those that did not?

b. Does SRBC differ on average between the birds that received supplemental carotenoids and those that did not?

c. What assumptions did you require in parts (a) and (b)?

Treatment CAROTCAROT CAROTCAROT CAROTCAROT CAROTCAROT CAROTCAROT CAROTCAROT CAROTCAROT CAROTCAROT CAROTCAROT

CAROTCAROT NONO NONO NONO NONO NONO NONO NONO NONO NONO NONO 1.7211.721 1.4541.454 1.2261.226 1.1981.198

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The Analysis Of Biological Data

ISBN: 9781319226237

3rd Edition

Authors: Michael C. Whitlock, Dolph Schluter

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