The same enzyme as in Problem 14 is studied in the presence of a different inhibitor (inhibitor B). In this case, two different concentrations of inhibitor are used. Data are as follows:

a. What kind of inhibitor is inhibitor B?
b. Determine the apparent Vmax and KM at each inhibitor concentration.
c. Estimate KI from these data.

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v[(mmol/L)min S (mmol/L) No in hi bi tor 3 mM in hi bi tor B 5 mM in hi bi torB 1.25 1.67 2.50 5.00 10.00 1.72 2.04 2.63 3.33 4.17 1.25 1.54 2.00 2.86 3.70 1.01 1.26 1.72 2.56 3.49