Wheeler and Mathews (J. Biol. Chem . 287:31218-31222 (2012)) reported the NAD+ and NADH concentrations in yeast mitochondria as 20 mm and 0.3 mM, respectively. Consider the malate dehydrogenase reaction, which is part of the citric acid cycle.

If malate concentration in yeast mitochondria is 0.4 mm, what is the maxi-mum concentration of oxaloacetate needed to make the reaction exergonic at pH 7.0 and 37° C?

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malateNADoxaloacetateNADHH +29.7 kJ/mol