You are constructing a velocity versus [substrate] curve for an enzyme whose KM is believed to be

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You are constructing a velocity versus [substrate] curve for an enzyme whose KM is believed to be about 2 μM. The enzyme concentration is 200 nM and the substrate concentrations range from 0.1 μM to 10 μM. What is wrong with this experimental setup and how could you fix it?
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Fundamentals of biochemistry Life at the Molecular Level

ISBN: 978-0470547847

4th edition

Authors: Donald Voet, Judith G. Voet, Charlotte W. Pratt

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