Question: You are constructing a velocity versus [substrate] curve for an enzyme whose KM is believed to be about 2 M. The enzyme concentration is 200

You are constructing a velocity versus [substrate] curve for an enzyme whose KM is believed to be about 2 μM. The enzyme concentration is 200 nM and the substrate concentrations range from 0.1 μM to 10 μM. What is wrong with this experimental setup and how could you fix it?

Step by Step Solution

★★★★★

3.43 Rating (172 Votes )

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock

To conclude we can say that the enzyme concentrat... View full answer

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Document Format (1 attachment)

930-M-S-B-C (1298).docx

120 KBs Word File

Students Have Also Explored These Related Biochemistry Questions!

The opening page of this chapter shows the titration curve for an enzyme. Is the average charge of the protein positive, negative, or neutral at its isoionic point? How do you know?

(a) Use the data in Problem 1 to construct a smooth graph of position versus time. (b) By constructing tangents to the x (t) curve, find the instantaneous velocity of the car at several instants. (c)...

A novel scheme for dissipating heat from the chips of a multichip array involves machining coolant channels in the ceramic substrate to which the chips are attached. The square chips (L c = 5 mm) are...

Hello i need help with the calculations and the drawing of the graphs. Basically the last picture where says laboratory report, the part of results. KINETICS OF A SINGLE SUBSTRATE ENZYME-CATALYSED...

PLEASE HELP!!! For a reaction that is taking place at equlibrium, which of the following is true? Neither the forward reaction nor the backward reaction are occurring. Only the forward reaction is...

Table 3: Absorbance values per minute for varied substrate concentrations with Inhibitor Time (min) Concentration (mM) 2.0 5.0 8.0 12.0 20.0 0 0 0 0 0 0 Absorbance 1 0.01 0.03 0.04 0.045 0.05 Table...

help asap....question 1 i need help with questine 1 & 2 and the graphs please Part B 1. Calculate the units of enzyme activity for the varying amounts of sub- strate. Calculate the substrate...

Hello, I need help on this Biomedical course homework. I would appreciate if you would me on this. Thank you. BME 201 - Spring 2024 - Homework #11: Name: Due: 4/19/2024 at 11:59 p.m. Remdesivir is an...

The enzyme lipase catalyzes the hydrolysis of esters of fatty acids. The hydrolysis of p-nitrophenyloctanoate was followed by measuring the appearance of p-nitrophenol in the reaction mixture: The...

solve for table 3.2 with the given data. you do not need to solve from assay 1 to 6, but at least show how you calculate one example of each column (corrected A410, p-nitrophenolate, p-nitrophenol...

Explain why is the centrifugal force talked about so much if it's not real?

A team member has a problem within the project, but isn't sure how to fix it. He shares this issue with the team to make sure everyone is aware of the challenge that came up. What Scrum core value...

Question 6 0 of 7 5 . Shannon files single and claims her daughter, Maya ( 2 5 ) . Her adjusted gross income ( AG 1 ) is $ 1 3 0 , 0 0 0 . What is the maximum amount $ hannon may potentially be able...

The fraction of defective integrated circuits produced in a photolithography process is being studied. A random sample of 300 circuits is tested, revealing 18 defectives. (a) Use the data to test the...

The Na+/glucose symport transports glucose from the lumen of the small intestine into cells lining the lumen. Transport of 1 glucose molecule is directly coupled to the transport of 1 Na+ ion into...

The classic demonstration that cell plasma membranes are composed of bilayers depends on the following kinds of data: Te membrane lipids from 4.74 109 erythrocytes will form a mono-layer of area...

The lipid portion of a typical bilayer is about 30 thick. a. Calculate the minimum number of residues in an -helix required to span this distance. b. Calculate the minimum number of residues in a...

6. The following accounts are reported for the Whitewasher Corp. for the end of fiscal year 2013 (all figures in millions): Cash = $12.0 Accounts Payable = $108.0 Inventories = $180.0 Total Current...

If b) is true (sample belongs to a population with a mean different than 82), what type of error are we making by not rejecting the null hypothesis in a)? Group of answer choices A)Error type II B)...

Ive had some trouble making this program work out. Write an original Java program to parse the words from a long String. The Java library offers similar functonality within its tokenizer classes, but...