A chromatographic procedure separates 4.0 mg of unknown mixture on a column with a length of 40 cm and a diameter of 0.85 cm.
(a) What size column would you use to separate 100 mg of the same mixture?
(b) If the flow is 0.22 mL/min on the small column, what volume flow rate should be used on the large column?
(c) If mobile phase occupies 35% of the column volume, calculate the linear flow rate for the small column and the large column.