Question: A chromatographic procedure separates 4.0 mg of unknown mixture on a column with a length of 40 cm and a diameter of 0.85 cm. (a)
A chromatographic procedure separates 4.0 mg of unknown mixture on a column with a length of 40 cm and a diameter of 0.85 cm.
(a) What size column would you use to separate 100 mg of the same mixture?
(b) If the flow is 0.22 mL/min on the small column, what volume flow rate should be used on the large column?
(c) If mobile phase occupies 35% of the column volume, calculate the linear flow rate for the small column and the large column.
(a) What size column would you use to separate 100 mg of the same mixture?
(b) If the flow is 0.22 mL/min on the small column, what volume flow rate should be used on the large column?
(c) If mobile phase occupies 35% of the column volume, calculate the linear flow rate for the small column and the large column.
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a b c Large load Small load 100 mg Large column diameter 40 mg 085 cm diameter Large column radius ... View full answer
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