A mixture of four -[32P]-labeled ribonucleoside triphosphates was added to permeabilized bacterial cells undergoing DNA replication in
Question:
(a) Why was the excess of unlabeled rNTPs added?
(b) How could you tell that radioactivity is being incorporated as ribonucleotides rather than as an alternative such as reduction to deoxyribonucleotides, followed by incorporation?
(c) What does this experiment tell you about the process of DNA replication?
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a This is a pulsechase experiment in which the large excess of nonradioative rNTPs red...View the full answer
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Related Book For 
Biochemistry Concepts and Connections
ISBN: 978-0321839923
1st edition
Authors: Dean R. Appling, Spencer J. Anthony Cahill, Christopher K. Mathews
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