Ice-cold ethanol is used to precipitate DNA. What is the significance of the temperature of the ethanol?

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Ice-cold ethanol is used to precipitate DNA. What is the significance of the temperature of the ethanol? Is this more important for shorter or longer DNA strands?

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Fundamentals of biochemistry Life at the Molecular Level

ISBN: 978-0470547847

4th edition

Authors: Donald Voet, Judith G. Voet, Charlotte W. Pratt

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Question Posted: Apr 30, 2016 02:53 PM

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Ice-cold ethanol is used to precipitate DNA. What is the significance of the temperature of the ethanol?

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Ethanol decreases the Tm of the DNA as well as decreas...View the full answer

Fundamentals of biochemistry Life at the Molecular Level

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