The following data on baker’s yeast in a particular medium at 23.4°C were obtained in the presence and in the absence of an inhibitor, sulfanilamide. The reaction rate (–r_S) was measured in terms of the oxygen uptake rate Q_O2, obtained as a function of oxygen partial pressure.
a. Assume the rate follows Michaelis–Menten kinetics with respect to oxygen. Calculate the maximum (i.e., Vmax), and the Michaelis–Menten constant KM.
b. Using the Lineweaver–Burk plot, determine the type of inhibition sulfanilamide that causes the O₂ uptake to change.

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Po* Qo (no sulfanilamide) Qo (20 mg sulfanilamide/mL added to medium) 0.0 00.0 0.5 23.5 1.0 33.0 1.5 37.5 2.5 42.0 3.5 43.0 5.0 43.0 00.0 17.4 25.6 30.8 36.4 39.6 40.0 *Po = oxygen partial pressure, mmHg; Qo = oxygen uptake rate, L of O per hour per mg of cells.