Question: 2. A fixed-bed adsorption column with a diameter of 2.0 cm and a bed height of 5.8 cm was used to isolate trypsin. The

2. A fixed-bed adsorption column with a diameter of 2.0 cm and

2. A fixed-bed adsorption column with a diameter of 2.0 cm and a bed height of 5.8 cm was used to isolate trypsin. The concentration of trypsin in the feed was 0.190 mg/mL. The flow rate of the feed is 0.109 cm/sec. The void volume fraction in the bed was 0.35. The bulk density of the adsorbent (in its hydrated state in the bed) is 1.03 g/cm 3. The volumes at breakthrough (0.1y) and exhaustion (0.9y) are 150 and 450 cm, respectively. (a) (b) Estimate the loading of the adsorbent for trypsin (mg/g) at breakthrough and at exhaustion for the feed concentration given. If you choose a breakthrough concentration of 0.01 mg/mL at 200 cm fraction of adsorbent bed utilized? 3, what is the

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