Question: Buffer 1 PNP (MM) at time (min) Initial velocity (uM/min) 10 20 30 A -8.09375 -7.46875 -7.15625 0.0469 B 0.421875 6.4375 13.078125 0.6328 C 10.109375

Buffer 1 PNP (MM) at time (min) Initial velocity (uM/min) 10 20 30 A -8.09375 -7.46875 -7.15625 0.0469 B 0.421875 6.4375 13.078125 0.6328 C 10.109375 23.390625 38 1.2538 D 11.046875 22.921875 36.125 1.3945 E 15.890625 34.25 52.375 1.8242 F 20.34375 41.28125 61.90625 2.0781 G 23.078125 44.875 65.890625 2.1406 H 25.5 47.6875 70.265625 2.2383

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock blur-text-image

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Students Have Also Explored These Related Mathematics Questions!

How to approach this problem? What do I need to do? I was confused. Please help. Given this information: The stationary phase is often some kind of solid medium, like silica gel or a resin. They...

Asking: Pick your four concentrations in the range of 10-75 M and calculate the dilutions youll need to perform from the stock solution to prepare them. This week in lab, we will be performing two...

please explain how to apply dilution factor here. Ty Make a dilution of PNP using your buffer as the diluent. Add 0.5mL of 1.25mM p-nitrophenol (PNP) to a 25 or 50mL beaker using a micropipette....

Scenario 2 should be answered given gel electrophoresis marks. Please give a guideline on how to answer this question. SCENARIO 1 IS JUST FOR BACKGROUND INFORMATION 2. Hypothetical protein analysis...

3.If you were to make a 12 mL extraction buffer, what volume of PMSF would you need to add? Why is PMSF added? 4.Calculate the volume of L-DOPA to add to get each of the concentrations used in the...

a) Describe the reaction that is being measured in this experiment. b) Describe your predictions regarding the protein content in each sample. c) Describe your predictions regarding the activity in...

solve for table 3.2 with the given data. you do not need to solve from assay 1 to 6, but at least show how you calculate one example of each column (corrected A410, p-nitrophenolate, p-nitrophenol...

Describe in practical terms how you would prepare 100 ml of 0.5 M Tris-HCl buffer (pH = 8.0) containing 0.1% SDS from the reagents listed on p. 8 Reagent Tris base Na2HPO4.7H2O Naci KH2PO4 FW (g/mol)...

1. Convert 3.8 nM to mM. 2. Convert 0.25 mM to mg/mL, assuming the molecular weight of the solute, a protein, is 47,300 g/mol. 3. You have a 0.25% (w/v) solution of the detergent SDS (288.4 g/mol)....

For many years, a company has paid all employees 1 week's wages as a year-end bonus. It is also company policy to give 2-week paid vacations. What accounting should be followed with respect to the...

Which of the following statements is CORRECT regarding federal estimated tax payments and withheld taxes for individual taxpayers? Quarterly estimated tax payments are considered as if they were...

Calculating Standard Deviation A stock had returns of 1 6 . 7 9 percent, 1 0 . 5 6 percent, 2 1 . 8 4 percent, and 1 3 . 3 9 percent for the past four years. What is the standard deviation of the...

Erie Company manufactures a mobile fitness device called the Jogging Mate. The company's labor standards for one Jogging Mate are as follows: Standard Hours Standard Rate per Hour Standard Cost 24...