Determine the amino acid sequence of an octapeptide with a composition of 2 ala, 1 arg, 2 tyr, 1 val, 1 asp, 1 met. ( NH4+ was detected in the acid hydrolysis.)

a) Partial acid hydrolysis yields the product whose structure is shown below.

b) Chymotrypsin treatment renders 2 tetrapeptides, each containing an ala.

c) Trypsin treatment of one of the tetrapeptides produces 2 dipeptides.

d) Treatment with CNBr of the same tetrapeptide gives a tripeptide and free tyr.

e) Treatment with Sanger's reagent of the other tetrapeptide generates DNP - asp.

f) The tetrapeptide that doesn't have methionine ends in tyrosine

g) Treatment of the original octapeptide with Sanger's reagent generates DNP-ala.

The peptide sequence (using single letter amino acid codes) is _____________________.

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Chymotrypsin cleaves at aromatics like Y To produce two tetrapeptides from an octa... View the full answer